Urine production and excretion has been one of the intricate problems investigated in the field of biomechanics. However, the mechanism that transports urine from the kidney into the bladder has not been fully understood. Engineering analysis of peristalsis in the ureter can be helpful in better understanding of function and abnormalities of this organ of the urinary system and also aid in the design of flow aided devices such as valves and stents to remove these abnormalities. In this paper, urine isolated bolus transportation in the ureter was simulated using ureteral anatomical data during peristalsis. Urine pressure distribution in the ureter, shear stress of ureteral inner wall, bolus dynamic deformations during its propagation and the effect of pressure difference Abstract- Urine production and excretion has been one of the intricate problems investigated in the field of biomechanics. However, the mechanism that transports urine from the kidney into the bladder has not been fully understood. Realization of peristalsis in the ureter may be helpful in better understanding of function and abnormalities of this organ of the urinary system and also aid in the design of flow aided devices such as valves and stents to remove these abnormalities. In this paper, urine isolated bolus transportation in the ureter was simulated using ureteral anatomical data during peristalsis. Urine pressure distribution in the ureter, shear stress of ureteral inner wall, bolus dynamic deformations during its propagation and the effect of pressure difference between the kidney and the bladder on the quantity of reflux and efficiency of urine transportation as a result of peristalsis, were investigated. A computational model was presented that used the tools of computational fluid dynamics, Arbitrary Lagrangian-Eulerian formulation, incompressible Navier-Stokes equations and adaptive mesh algorithm in the fluid domain. In the structure domain, it utilized Arruda-Boyce non-linear model and contact condition. The major benefits of this model comparing to previous studies were that the ureteral wall displacements were not pre-determined during peristalsis and luminal pressure variations influenced on it. Finite element equations of fluid and structure were solved using fluid-structure interaction method (FSI) and direct coupling. Results of this research showed that the proximal portions of ureter were under higher magnitudes of shear stress. Moreover, increase of the bladder pressure magnified the quantity of ureteropelvic reflux in the case of dysfunction of ureteropelvic junction, and resulted in a higher peristaltic efficiency transporting ureteral bolus into the bladder.