مهندسی مکانیک مدرس

مهندسی مکانیک مدرس

طراحی یک سامانه میکروسیالی دو مرحله‌ای برای جداسازی اسپرم در بیماران مبتلا به بی‌نطفگی، جهت به‌کارگیری در لقاح آزمایشگاهی

نوع مقاله : پژوهشی اصیل

نویسندگان
محمدرضا ملکی 1
پیمان مصدق 1
رضا نصرتی 2
احمدرضا پیشه ور 1
1 دانشکده مهندسی مکانیک، دانشگاه صنعتی اصفهان، اصفهان، ایران
2 دانشکده مهندسی مکانیک و هوافضا، دانشگاه موناش، ملبورن، استرالیا
10.48311/mme.2025.27739
چکیده
ناباروری یکی از چالش‌های رو‌به‌رشد جهانی است که حدود %۱۵ از زوج‌ها را درگیر کرده و در نیمی از این موارد، عوامل مردانه نقش اصلی را ایفا می‌کنند. شدیدترین شکل ناباروری مردان، بی‌نطفگی غیرانسدادی (NOA) است که در آن تولید اسپرم به‌شدت کاهش می‌یابد. بازیابی اسپرم از نمونه‌های بافت بیضه در این بیماران، فرآیندی زمان‌بر و وابسته به تجربه اپراتور است و حضور سلول‌های زمینه‌ای و بقایای بافتی، شناسایی اسپرم‌ها را دشوار می‌سازد. در این مطالعه، یک سامانه‌ی میکروسیالی دو مرحله‌ای غیرفعال طراحی و شبیه‌سازی شد که ترکیبی از یک ماژول مارپیچ اینرسی و یک ماژول DLD است. در مرحله نخست، ماژول مارپیچ با بهره‌گیری از نیروهای اینرسی و گردابه دین، ذرات بزرگ‌تر را از جریان اصلی حذف کرده و از انسداد کانال‌ در مرحله دوم جلوگیری می‌کند. در مرحله بعد، ماژول DLD با تفکیک‌پذیری بالا، سلول‌های پس‌زمینه باقی مانده را حذف و جداسازی اسپرم را تکمیل می‌نماید. نتایج شبیه‌سازی نشان می‌دهند که ذراتی با قطر کمتر از ۴٫۷ میکرومتر، که به‌عنوان نماینده‌ای از اسپرم‌ها و گلبول‌های قرمز باقی‌مانده در نظر گرفته شده‌اند، به‌طور مؤثر از سلول‌های بزرگ‌تر زمینه جدا می‌شوند و بدین ترتیب از انسداد کانال‌های میکروسیال در مرحله بعدی جلوگیری می‌گردد. با توجه به توانایی بالای سامانه DLD در جداسازی سلول‌ها با تفکیک‌پذیری بالا، این سامانه قادر است سلول‌های اسپرم را با راندمان بالای %95 از گلبول‌های قرمز باقی‌مانده تفکیک کند.
 
کلیدواژه‌ها
بی‌نطفگی
ناباروری
اسپرم
میکروسیال
جداسازی

موضوعات

عنوان مقاله English

Design of a Two-Stage Microfluidic System for Sperm Isolation in Azoospermic Patients for Application in In Vitro Fertilization (IVF)

نویسندگان English

Mohammadreza Maleki 1
Peiman Mosaddegh 1
Reza, Nosrati 2
Ahmadreza Pishevar 1
1 Department of Mechanical Engineering, Isfahan University of Technology, Isfahan, Iran
2 Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, Australia
چکیده English

 
Infertility is an increasingly prevalent global issue, affecting approximately %15 of couples, with male factors accounting for half of these cases. The most severe form of male infertility is non-obstructive azoospermia (NOA), in which sperm production is drastically reduced. Sperm retrieval from testicular tissue samples in these patients is a time-consuming process that relies heavily on the operator’s expertise. The presence of background cells and tissue debris further complicates the identification of sperm. In this study, a two-stage passive microfluidic system was designed and simulated, integrating an inertial spiral module with a deterministic lateral displacement (DLD) module. In the first stage, the spiral module utilizes inertial and Dean vortex forces to remove larger particles from the main flow, thereby preventing channel blockage in the second stage. In the subsequent stage, the high-resolution DLD module removes the remaining background cells, completing the sperm separation process. Simulation results demonstrate that particles smaller than 4.7 μm in diameter, corresponding to sperm cells and residual red blood cells, are efficiently separated from larger background cells, effectively preventing clogging in downstream microfluidic channels. Given the DLD module’s high resolution, the system can isolate sperm cells from residual red blood cells with more than %95 efficiency.
 

کلیدواژه‌ها English

Azoospermia
Infertility
Sperm
Microfluidics
Separation
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