Phenomenon of dispersion and deposition of nano- and micro-particles in turbulent flows been focused in the past decades. In this paper, particle dispersion and deposition in gas-particle two-phase turbulent flow inside a two-dimensional channel with rectangular artificial roughness is studied using an Eulerian–Lagrangian method. The RSM turbulence model with enhanced wall treatment was used to simulate the anisotropic turbulent gas phase flow. The gas phase flow predictions were validated by comparing the results with available experimental data for a fully developed asymmetric turbulent channel flow. In discrete phase, Lagrangian approach was applied for particle tracking. The Lagrangian equation of particle motion includes drag, gravity, Saffman lift, and Brownian forces. The particle phase simulation results were validated by comparing the present work with available equations and valid data for a gas particles turbulent flow inside a two-dimensional smooth channel. The gas phase simulation results show that by increasing the artificial roughness height, a recirculation region which is created in the space between two ribs, becomes larger. The particle phase results show that the rate of deposition in the channel with artificial roughness is a function of gravity force and flow pattern in the space between two ribs. The rate of deposition for small particle is affected significantly by gas flow pattern in the space between two ribs. However for large particles the gravity force is more dominant.