Boiling heat transfer is one of the most applicable heat transfer processes within the industry. In this paper, the pool boiling heat transfer of Fe3O4 /water nanofluid (ferrofluid) in atmospheric pressure has been analyzed, experimentally. The nanofluid in this study, has been synthesized in a single step and retains high stability. The replication and accuracy of the testing machine has been studied for deionized water for three times, indicating an appropriate concordance with the literature. Considering different volume concentrations of the nanofluid has revealed that boiling heat transfer in high concentrations decreases with an increase of concentration, while it rises with the increase of concentration in low concentrations. Hence, boiling heat transfer coefficient in 0.1% volume concentration nanofluid has been measured to be the optimum value which increases up to 43%. The roughness of boiling surface was varied with the deposition of nanoparticles in various conditions of nanofluid concentration, and heat flux. It is noteworthy that in the present research, the effects of surface roughness changes due to nanoparticles deposition and the impact of passing time on boiling process have been investigated, for the first time. Therefore, several experiments have been designed in order to study the change of nanoparticles deposition due to the change of nanofluid concentration and boiling surface heat flux. The results indicate that boiling heat transfer of deposited surfaces at low heat fluxes decreases; while it rises at high heat fluxes.