One of the important elements of the novel that constitutes its main form is the element of plot. The plot consists of a structure consisting of start, expansion, suspension, climax, untying, and ending. Knowing this structure allows the reader to experience the story well and understand it through a specific time process. Contemporary novelists, given the experience they gained from the new world, They found that in writing the story, the sequence of the plot structure could be broken and disturbed. In such stories, due to the abandonment of accepted traditions of art and the creation of new narrative styles, the events of the story are intertwined and the boundary between beginning and end is not defined. It also sometimes happens that the characters of the story invite the reader to implicitly play a role in the ending of the story. In the present study, based on the descriptive-analytical method, an attempt is made to make a comparative study of nonlinear plot in the novel " Land of the Missing" by Elias Khoury and the novel "I am not a tiger" by Mohammad Reza Safdari. Given that in these two works, traces of modern and postmodern fiction can be seen

Introduction: Nowadays, bone tissue repair with increasing bone disorders and injuries have special importance. Bone tissue engineering provided specific solutions to these problems. The present study was conducted with the aim of purification of recombinant fusion peptide containing hydroxyapatite affinity tag using the ceramic chromatography column.
Material & methods: In this study, a fusion peptide was designed which at one side comprised the heparin-binding domain sequence, which can be attached to various types of growth factors involved in tissue repair and entrap these factors at the site of the lesion. On the other side, it contained a tag, which included a sequence derived from a laboratory study based on phage expression. The reason for keeping the sequence of this tag is to attach the peptide to the scaffold containing hydroxyapatite and purifying the recombinant peptide by the hydroxyapatite column. Therefore, the gene sequence was optimized and synthesized for expression in the prokaryotic host of E.coli strain BL21. Then the gene sequence was subcloned by double digestion with the SacI and BamHI enzymes into the expression vector of pET-21a(+). The expression of the recombinant peptide was investigated by SDS-PAGE and western blot. In order to optimize the purification conditions, two-step purification was carried out by applying fundamental changes in the main work method of the manufacturer company and was purified with acceptable purity. Finally, the existence of peptide assemblies was investigated by the SLD method.
Finding: The results of PCR cloning, enzymatic digestion using SacI and BamHI enzymes and sequencing indicated the accuracy of the cloning process. On the other hand, expression of the fusion peptide was confirmed by SDS-PAGE and Western blot techniques, and its migration onto the gel resulted in a band cleavage of about 12 kDa. Changes made to the manufacturer's workflow allowed the purification process to be optimized and the results of the DLS method showed the purity of the purified peptide.
Conclusion: The results indicate the desirable expression and remarkable purity of the fusion peptide designed in this study.

The purpose of this study was to produce red beetroot pulp powder using foam-mat drying technique and to investigate the effect of foam thickness on drying kinetics and physicochemical and microstructural properties of the resulting powder. To prepare foam, ovalbumin as foaming agent at a concentration of 2% (w / v) was added to the red beetroot pulp and was stirred for a specific time and speed. Prepared foam was spread on aluminum plates at thicknesses of 5, 6 and 7 mm and then dried in a hot air cabinet dryer at 50 ºC and constant air velocity of 1 m/s. Results showed that increasing foam thickness from 5 to 7 mm had no significant effect on moisture content and moisture ratio but significantly (p <0.05) reduced the drying rate by about 29.63%. The effective moisture diffusion coefficient was significantly affected by the foam thickness and ranged between 5.550 × 10⁻⁹ and 7.388 × 10⁻⁹ m²/s. Also, with increasing foam thickness, due to increasing drying time and more denaturation of proteins, an increase in Carr index and Hausner ratio was observed, resulting in a decreasing trend in the flowability of the powders. Bulk and tapped densities of powders were significantly decreased due to the change in moisture content, caused by the increase in foam thickness. Thickness had no significant effect on water solubility index and water absorption index. The microstructure analysis of the produced powders by field emission scanning electron microscope showed that with increasing foam thickness, cracking and surface roughness of powder particles increased. The results of this study and further studies can lead to optimization of red beetroot powder production as a natural food coloring and better preservation of its nutritional and functional properties.