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measurement accuracy. The angular response of the sensor describes the relationship between flow velocity vector and heat transfer from the sensor, which is determined by a sensitivity function. In this paper, two sensitivity functions, namely cosine law and Hinze equation, have been studied using wind tunnel experiments to evaluate the effect of various parameters such as flow conditions (velocity and direction), probe aspect ratio (l/d) and probe operational condition (sensor temperature) on the range of applicability of cosine law and magnitude of the sensitivity coefficient, k. Results show that the angular range of applicability of cosine law depends on flow and probe conditions. At 1% measurement error, the range of applicability of cosine law for flow measurements of velocities exceeding 10 m/s was found to be in the range of ±30º. Moreover, at geometrical ratios higher than 600, two-dimensional flow measurements using the cosine law presents results with acceptable accuracy. In addition, the sensitivity coefficient is completely dependent on flow condition and probe aspect ratio, and its value decreases with increase in flow angle and velocity and reduction in probe aspect ratio. The results of this research can be used in the selection and proper design of probes for two-dimensional flow measurements using hot wire anemometers.

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The influenza A virus is of global concern for the poultry industry, especially the H5 subtype as it has the potential to become highly pathogenic for poultry and mankind. Recently, plant expression systems have gained interest as an alternative for the production of vaccine antigens. The goal of the present study was to investigate the possibility of expressing the HA1 protein in Nicotiana tabacum via agroinfiltration. In this study, the Hemagglutinin type 1 (HA1) of a high pathogenic avian influenza virus of the H5N1 subtype was synthesized and transiently expressed in Nicotiana tabacum. To examine the possibility of expressing the HA1 protein in N. tabacum, a cDNA fragment encoding the HA1 gene was synthesized de novo, modified with a Kozak sequence, a C-terminal hexa-Histidine (6His) tag, and an endoplasmic retention signal (KDEL). The construct was cloned into vector and the resulting - HA1 plasmid was agro-infiltrated into N. tabacum. The relative gene expression of recombinant plant-produced HA1 was measured by quantitative real-time PCR. Guided by the gene expression profile, HA1 protein was extracted at 3 dpi and subsequently purified utilizing the 6His tag. A recombinant HA1 protein was immunogenically detected by conjugated polyhistidine antibody in western blot, dot blot and ELISA assay. In order to verify the right conformation of HA1 produced in plants, western blot was also done using mouse monoclonal anti-influenza A virus (H5N1/HA1) [2B7]. The results of Real Time PCR assay indicated that the foreign gene was transcribed in transfected leaves. Migration size of protein was detected at 45 kD by Western blotting and demonstrated no discrepancy compared to the positive control (HA1). ELISA results showed that the HA1 was expressed in the transfected leaves in high level as the yield of recombinant protein was 8.8 % of TSP and the yield of purified HA1 was 0.16 g purified protein per kg fresh weight of leaves. This is the first research about the transient expression of the tobacco-made HA1 protein where a synthetic sequence was used for its expression. Here, the efficacy of agro-infiltration for expression of HA1 antigen in tobacco was illustrated. Agro-infiltration expedites the process of recombinant antigens expression in plant tissues. Accordingly, our results provide great opportunity for the exploration of transiently plant-manufactured HA1 as vaccine candidate.

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abrasive water jet is one of the most popular cutting methods today due to its ability and unique features such as the ability to cut complex shapes as well as the wide range of materials and non - creation of thermal distortion at the cutting site. The accuracy of the process is mainly due to selecting the cutting parameters. The sheet used in this study is aluminum 7075 with a thickness of 25 mm. In this research, for water jet pressure, forward speed and nozzle distance to the surface of the workpiece, three surfaces and for the impingement angle of two surfaces were considered. In order to investigate the quality of the cut - off area, the effect of process parameters on the high and low width of the cut section and also the cut - surface slope is investigated. Finally, the cut gap created by this process has special geometrical features that in some cases is the limitation of the process. The results showed that the change in the direction of the water jet from the perpendicular to the surface of the work piece, although the width of the cutting crack cannot be changed, the surface quality decreases and the cutting surface slope increases.