Showing 35 results for Sattari
Volume 0, Issue 0 (Articles accepted at the time of publication 2024)
Abstract
The positive role of reflective teaching and well-being as means of fostering teaching quality has mainly remained at the level of speculation and there is little empirical evidence to illustrate their impact on enhancing professional development. To fill this existing gap, this study examines the contribution of reflection and psychological well-being as predictors of professional development. Adopting a mixed-methods approach, 350 English language teachers were selected randomly and participated in a survey. Following that, six teachers through purposeful sampling participated in a focus group interview to investigate the relationship among the aforementioned variables. The correlational analysis confirmed the positive relationship among these three constructs and a structural equation modeling indicated that both reflection and well-being significantly predicted professional development; however, well-being was a stronger predictor compared to reflection. The qualitative analysis of data revealed four main themes contributing to professional development among teachers. The pedagogical implications are also elaborated and discussed.
Volume 4, Issue 4 (winter 2024)
Abstract
Problem: Residents of residential complexes in urban areas face all kinds of sounds every day. Most of these hundreds are very noisy and painful in traffic centers.
Target: The aim of the current research is to evaluate the satisfaction of the residents of Tabriz railway neighborhood from the perspective of environmental acoustic comfort.
Method: The current research method is descriptive-analytical with practical purpose. The statistical population of the research is 32,936 residents of the Tabriz railway neighborhood. The sample size was 380 people using Cochran's formula. For the validity of the questions, face validity was used and Cronbach's alpha was used for reliability. To analyze the data, structural equation method and TOPSIS and FTOPSIS techniques from Spss and Amos software were used.
Result: The results showed that among the factors affecting the acoustic comfort of the residents of the Tabriz railway neighborhood, the physical index had the greatest impact, followed by the acoustic and social indicators. Also, the results showed that in terms of the ranking of the koi in terms of acoustic comfort in terms of noise pollution, Ittahad alley got the highest noise pollution, followed by Niloufar alley and Shaghaig koi, the second and third respectively As a result, with proper design and use of form, facade and materials in terms of architectural acoustics, noise pollution can be reduced to a great extent in Tabriz Railway neighborhood and help to improve the quality of acoustic comfort of the residents.
Volume 5, Issue 4 (winter 2024)
Abstract
Problem:One of the most important elements of urban spaces is urban furniture, which has special importance for citizens.Urban furniture,in addition to providing services to citizens,makes them feel comfortable in urban spaces, and by strengthening the level of satisfaction,it can lead to more interaction with other citizens.Aims:Considering the importance of observing standards in the design of urban furniture and the lack of attention to this issue in Iran, the main goal is to investigate the considerations and criteria for designing urban furniture in urban spaces.Methodology:After conducting documentary studies, first,different definitions of urban furniture were examined,then its classification in the world and Iran and its criteria were studied.Findings:The results of this research show that urban furniture design can be examined in three dimensions:1-Grammatical dimensions: this dimension examines the system and structure of signs,2-Functional dimensions:it analyzes the relationship between a sign and its user and 3-Semantic dimensions:it deals with the study of meaning, change of meaning, and principles governing the relationships between sentences and words and their meaning.Also,It can be said that in terms of how to pay attention to design considerations and criteria, there can be two modes;1-Standard-oriented and uniform design, in which all considerations and criteria are taken into account in the design process and 2-The design of a case that is based on special conditions and based on the location and platform of the furniture and by observing the appropriate considerations and criteria,the subject of creativity,styles,and artistic theories can find more expression and strength in this category of furniture.
Volume 6, Issue 1 (6-2017)
Abstract
Growth, sex ratio and age of 188 specimens of Tench, Tinca tinca, from Anzali wetland were recorded during Nov. 2013 to June 2014. The age range of fish was 0+ to 6+ years. Male to female sex ratio was 1:1.7 that differed significantly (P<0.05). Maximum total length (TL) and weight (W) was 40.7 cm and 1000g, respectively. The b value of the length-weight relationship was 2.70 that showed negative allometric growth (p<0.05). The growth performance index (ϕ) was calculated as 2.61. The estimated von Bertalanffy growth parameters were (L∞= 47.48 cm, k= 0.18 yr- and t0= -1.07 yr). Tmaxwas calculated 16.41 years. Also infinity weight (W∞) was estimated as 1261.43 g. The present investigation provides basic information about population structure of Tench (T. tinca) in Anzali wetland.
Volume 8, Issue 1 (6-2006)
Abstract
Purpose: In this study the inhibitory effect of Eucalyptus extracts as a natural and herbal antibacterial substance was evaluated against Pseudomonas aeruginosa strains (8821M and ATCC27853).
Material and Methods: The MIC (Minimal Inhibitory Concentration) of alcoholic and aquatic extracts of Eucalyptus was determined using the tube and agar dilution methods. The growth rate of Pseudomonas aeruginosa in sub-MIC concentration of extracts was compared with the controls. Phenol coefficients of extracts were determined by the Ridal- Walker method.
Result and Discussion: The MIC was 1:8(3.2 mg/ml) fold of the alcoholic extracts and 1:4(17.5 mg/ml) fold of the aquatic extracts. In the sub-MIC concentration of extracts, by increasing the Eucalyptus extract concentration, the growth rate was decreased. Phenol coefficients of the alcoholic and aquatic extracts were evaluated to be 0.0381 and 0.019, respectively.
Conclusion: Results of this study indicated that crude aquatic and alcoholic extracts of Eucalyptus have inhibitory effects on the growth of Pseudomonas aeruginosa and in the sub- MIC concentration of extracts this value decreases. Overall, the plants indicated a wide range of antimicrobial activities which can lead to the detection of new antibiotics against resistant bacteria.
Volume 8, Issue 4 (2019 2019)
Abstract
Aims Sacred architecture is a reflector of divine beauties, and it is an illustrative example of the combination and association of symbolic forms with our deep beliefs and this connection can be expressed in the deepest mysterious layers of the altar. The altar is the most important part of the religious building. In the history of architectural decorations in Islamic art, altar has always embedded the best and most valuable examples in terms of style and in terms of motifs as well as calligraphy values. The Aljaito altar, in terms of the art of Stucco used in it, is in the rank of the most beautiful works of the Ilkhani era. In this research, the study of the herbal designs of the altar, the characteristics, and generalities of the motifs and the patterns governing them are the aim of the research.
Instruments & Methods Through library and field studies, we have identified the decoration of the altar of the Jameh Mosque of Isfahan. The research method is descriptive-analytical and uses architectural software.
Findings & Conclusion The following results were obtained by examining the designs and decorations of Aljaito altar: 81 patterns of herbal design were extracted from the altar designs, with a range of 28% horizontal rectangle, 22% circle, 21% pentagon, 15% vertical rectangular, 7% star, 6% square, and 1% Shamseh; the majority of it included horizontal rectangle. Also, the dominant line in Aljaito altar designs adhered to 100% of the curve pattern, and 85% were completely non-symmetrical and 15% was vertical to the axis in the herbal designs.
Volume 11, Issue 0 (بهار و تابستان 87- 2008)
Abstract
Objective: Ajowan is an annual herbaceous essential oil of Carum copticom. The main components of the oil are Tymol, β-pinene, γ- terpinene and Sabinene.The fruit oil of Carum copticum has been reported to have several therapeutic effects including anti fungal, anti bacterial and anti viral,... Candida albicans is an opportunistic fungus and transforms into pathogenic form in favorable conditions, causing fungal diseases.
Materials and Methods: In this study, essential oil and alcoholic extract of Carum copticum were gained and Microdilution Broth method were used for detection of minimum inhibition concentration (MIC) and minimum fungicide concentration (MFC) of 11 clinical isolates of Candida albicans and Standard strain (PTCC50-27).
Results: Results show that MIC for essential oil is 0.43 µg/ml, 0.87 µg/ml and for alcoholic extract is 3.51 µg/ml, 7.03 µg/ml, 1/75 µg/ml. Thus, it seems that Carum copticum could inhibit Candida albicans growth by a similar mechanism which occurs by Fluconazole (FLZ). In general, the results obtained in this study indicate that Carum copticum has potential values for growth inhibition of Candida albicans in vitro.
Conclution: In recent years, systemic fungal infections due to Candida species have been received major consideration about inducing mortality in nosocomial patients because of increasing in immunocompromised disorders such as AIDS and hematological disorders as well as long term use of
Phlebotomus perfiliewi transcaucasicus sandflies.
Conclusion: This is the first report on natural infection of sand flies to L. donovani in the country and since the ecology and biology of L. donovani differs extensively from L. infantum, it is necessary to perform further studies to highlight the role of L. donovani in epidemiology of VL in the region and country.
Volume 12, Issue 1 (1-2009)
Abstract
Objective: Listeria monocytogenes is a facultative, gram-positive bacterium which is found in soil, water, decaying vegetables, raw milk, and contaminated dairies. Listeria monocytogenes causes listeriosis. Listeriosis is a zoonosis disease, which transfers from animals to humans by animal feces and contaminated dairies. Listeriosis causes the flu like disease or self-limited enteritis, but it leads to serious disease in elderlies, new-borns, pregnant women and immunocompromised persons.
If pregnant women are infected by Listeria monocytogenes, the newborn probably will be miscarried, prematured or stillborn.
For the importance of the bacteria on pregnant women’s and newborn’s health, there are so much concentration and studies on it.
Culturing of the bacteria is so difficult and time-consuming and it needs at least 5 days to confirm.
Our goal in this survey was to develop an PCR based molecular method for fast detection of the bacteria from the vaginal samples.
Materials and Methods: In this survey 100 vaginal samples were examined. All of the samples were cultured and assayed with PCR method.
Results: Among them, 7 samples for culture and 36 samples by PCR were positive for Listeria monocytogenes.
Conclusion: In this study we showed that the PCR is a faster, more accurate and sensitive than culture method for the detection of Listeria monocytogenes in vaginal samples.
Volume 12, Issue 1 (1-2009)
Abstract
Objective: Methicillin-resistant Staphylococcus aureus is a major cause of nosocomial and community-acquired infections. Aminoglycosides are potent bactericidal agents that are often used in combination with either a β-lactam or a glycopeptide, especially in the treatment of staphylococcal endocarditis. The main mechanism of aminoglycoside resistance in staphylococci is drug inactivation by cellular aminoglycoside-modifying enzymes.
The main aim of the present study is determining the prevalence of ant(4′)-Ia gene encoding one of the most important aminoglycoside-modifying enzymes and simultaneous detection of mecA gene responsible for methicillin resistance in clinical isolates of Staphylococcus aureus by Multiplex-PCR method.
Materials and Methods: A total of 100 clinical S. aureus isolates were collected from Shariati and Baqiatollah hospitals in Tehran, then antibiotic susceptibility pattern of strains were determined by disk diffusion method using penicillin, oxacillin, vancomycin, tetracycline, erythromycin, gentamicin, tobramycin, amikacin, netilmicin and kanamycin disks, considering CLSI principles. Using agar dilution method the MIC for oxacillin, gentamicin, tobramycin and amikacin were also determined. In order to detect resistance genes, ant(4′)-Ia and mecA, two pairs of specific primers were used and their prevalence was determined by using a Multiplex-PCR method.
Results: All strains were resistant to penicillin (100%) and after that the highest rate of resistance was observed against kanamycin (68%), tetracycline (61%), erythromycin (56%), tobramycin (53%), gentamicin (52%), amikacin and oxacillin (48%) and netilmicin (22%), respectively. All of the strains were also susceptible to vancomycin. In agar dilution method 50% of strains were oxacillin resistant and 49%, 45% and 51% of the strains showed resistance toward gentamicin, amikacin and tobramycin, respectively. Thirty-seven percent of the strains also showed high-level gentamicin resistance with MIC of ≥128µg/ml. In Multiplex-PCR method 53% of the strains possessed mecA gene and 58% of
the strains were ant(4′)-Ia positive.
Conclusion: Results obtained by phenotypic and genotypic antibiotic susceptibility determination tests show that there is a statistically meaningful relationship between methicillin resistance and aminoglycoside resistance in MRSA strains (P<0.05).
Volume 12, Issue 2 (summer 2024)
Abstract
The concept of linguistics is closely associated in contemporary literature with Reza Barahani and it comprises one of the most significant postmodern poetic movements. It emphasizes the importance of language over meaning and encapsulates poetry within the realm of language and linguistic forms. While this concept is linked to Barahani's name in terms of its foundations and poetic style, the principles and foundations of this theory can be traced in the postmodern poetry of other countries around the world. In Arabic poetry, the Lebanese modernist and surrealist poet Wadih Sa'adeh is a case worth investigating in this regard. The theoretical principles of the concept of linguistics were elucidated by Barahani, and the collection of poems "Khatab be Parvaneh-ha" (To the Butterflies) was composed and published by the poet under this theory. This article, using a descriptive-analytical method, aims to conduct a comparative study of the poems of Reza Barahani and the contemporary Lebanese poet Wadih Sa'adeh, relying on this theory, namely Barahani's theory of linguistics, to accurately and systematically explore the similarities and potential for critical analysis of the poems of a Lebanese poet with the concept of linguistics. This article has endeavored to uncover and reveal the extent of the two poets' attention to language and its formal, figurative, and purely linguistic features. The basis of the analysis is the American school of
Volume 12, Issue 4 (10-2010)
Abstract
Objective: Pseudomonas aeruginosa is the major cause of septicemia and wound infection in burned patients. Immunotraphy is the best practical way for prevention and treatment of these infections. Flagella as one of the most important bacterial virulence factors has important role in attachment, motility, chemotaxis and TLR-5-dependent immune response so that it propounded as a vaccine candidate. Production of anti-flagellar antibodies and evaluation of its protective effects in burned induced infection of mice was the main aim of this study.
Materials and Methods: In the first step, flagellar antigen prepared by ultra-centrifugation. Anti-flagellar antibodies produced in rabbit and its impurity separated by absorption technique. Specification of the obtained antibodies for flagellar antigen was investigated via agglutination test. After determination of LD50 in a known strain, different dilutions of anti-flagellar antibodies injected in burned mice for passive immunization. The rate of bacterial spread from burn site was determined by quantification assay of bacteria in skin and liver. In this study, clinical isolate and PA103 in addition to ATCC 27853 strain were used for agglutination test.
Results: H-antiserum reduced mortality of burned mice challenged with ATCC 27853 strains about 80%. Counting of bacteria in the skin and liver showed that the number of bacteria in immunized mice, in contrast with control group, was significantly low.
Conclusion: The results of this study showed that anti-flagellar antibodies of Pseudomonas can inhibit invasion of Pseudomonas and facilitate its opsonization, so these antibodies have protective effects in burned wound infections.
Volume 13, Issue 1 (1-2010)
Abstract
Objective: Verotoxin is a member of Shiga toxin family. This family contains AB protein toxins with an enzymatic (A) and a binding (B) compartment. Cells that have receptor (Gb3) are sensitive to cytotoxic effects of toxin. It has been shown that various tumor cells have Gb3 receptor and are selectively sensitive to apoptotic effect of verotoxin. Studies on tumor cell lines and laboratory animals have shown antineoplastic and antiangiogenesis effects of this toxin. The aim of this study was comparison of cytotoxic effect of verotoxin 1 on two cell lines: Vero (gold standard for evaluation of cytotoxic effect of Verotoxin) and Raji (a cell of a cultured line of lymphoblastoid cells derived from a Human Burkitt's lymphoma patients).
Materials and Methods: The toxigenic strain was cultured and the production of toxin was evaluated by reverse passive latex agglutination test. Verotoxin 1 was purified by affinity chromatography. Vero and Raji cell were treated with serial dilutions of toxin, and viability was evaluated by MTT test.
Results: Our result indicated that Verotoxin has cytotoxic effect on Raji cell lines. This effect is directly related to toxin concentration. Differences on cytotoxicity of toxin on Raji cells at 1:4-1:128 dilutions in relation to cytotoxicity of toxin on Vero cells at the same dilutions were considered statistically significant (P<0.05). But difference of cytotoxicity of toxin at higher dilutions was not significant.
Conclusion: Our results revealed that Verotoxin has cytotoxicity on Vero and Raji cells, and this
effect on Vero cells is more than Raji cells (P<0.05).
Volume 13, Issue 2 (Number 2 - 2011)
Abstract
Gas chromatography (GC) and gas chromatography mass spectrometry (GC-MS) were employed to determine the chemical composition of essential oil obtained from dry fruits of Carum copticum. Thymol (36.7%), -terpinene (36.5%) and -cymene (21.1%) were found to be the major constituents of the oil. The anti-bacterial activities of the oil were mainly investigated against food poisoning bacteria (Salmonella thyphimorium, Pseudomonas aeruginosa, Entero-pathogenic Escherichia coli, and Staphylococcus aureus) by broth microdilution and agar diffusion methods. The oil exhibited significant anti-bacterial activities against all the examined bacteria. In conclusion, the results of this study showed that the oil of Ajowan is rich in monoterpenes and it may be used as a natural anti-bacterial agent in drug and food industries.
Volume 13, Issue 2 (6-2024)
Abstract
This study aimed to produce a smart infection warning film based on chitosan and gum arabic containing anthocyanin (0.5, 0.75, and 1 g) as a wound infection warning. Chitosan/gum film was prepared in a ratio of 1:2 with different doses of anthocyanin pigment (0.5, 0.75, and 1 g), and the control sample was considered without anthocyanin. The prepared film was evaluated on Pseudomonas and Mannitol salt agar media. To evaluate the functional properties of the prepared film, the amount of moisture, solubility, thickness, and water absorption of the treatments were measured. The lowest and highest film moisture content were in the control treatment, and the treatment contained 0.75% anthocyanins, respectively. As the percentage of anthocyanin increased, the thickness and solubility of the samples decreased significantly. Regarding the water absorption test, the highest amount was related to the treatment containing 1% anthocyanin, and the lowest amount belonged to the control treatment. To evaluate the effect of chitosan or gum film containing anthocyanin on the culture medium when the bacteria were well grown, chitosan or gum biofilms containing different concentrations of anthocyanin were cut to 1.1 cm and cut into It was placed on the culture medium for 60 minutes, and the color change of the films was checked with a colorimeter. The highest rate of color change of films in culture medium containing Pseudomonas aeruginosa was obtained in the treatment containing 0.5%; however, in the case of Pseudomonas aureus, the highest color change was observed in the treatment containing 1%. Finally, the best film in terms of physical characteristics is the 1% treatment, and in terms of color change in response to the growth of Pseudomonas, it is 0.5%, and for Staphylococcus aureus, it is 1%.
Volume 13, Issue 3 (8-2010)
Abstract
Objective: Escherichia coli is the most prevalent etiologic agent of urinary tract infections which is the cause of about 80% of cases. Enzymatic inactivation of aminoglycosides by aminoglycoside-modifying enzymes is the main mechanism of resistance to these antibiotics in Escherichia coli. The aim of this study was the detection of aac(3)-IIa gene among aminoglycoside resistant clinical isolates of E. coli using PCR method.
Materials and Methods: After collection of 250 clinical isolates of E. coli, antibiotic susceptibility patterns of isolates were determined by disk diffusion method for gentamicin, amikacin, tobramycin, kanamycin and netilmicin by considering the CLSI principles. Chromosomal and plasmid DNA of the isolates were extracted using DNA extraction Kits and PCR method was used for detection of the aac(3)-IIa gene.
Results: Results show that 96% of E. coli isolates were resistant to tobramycin, 90% resistant to kanamycin, 82% resistant to gentamicin, 30% resistant to netilmicin and 8% resistant to amikacin. aac(3)-IIa gene was detected in 54.83% of E. coli isolates.
Conclusion: Because of high prevalence of resistance toward aminoglycoside antibiotics which is due to its transfer among bacteria by transferable elements such as transposons and plasmids. Therefor, tracing transfer routs among different bacteria is very important.
Volume 14, Issue 1 (1-2011)
Abstract
Objective: Shigellosis is one of the most common causes of morbidity and mortality in children with diarrhea in developing countries. It is essential to assess the antibiotic resistance patterns of these bacteria. ipaH gene is one of the virulence factors which can be used for detection of Shigella spp.
Materials and Methods: Total of 100 isolates of Shigella were collected from different provinces of Iran. This isolates were characterized by biochemical tests and serological tests using polyclonal antisera for 4 species of S. dysenteriae, S. sonnei, S. boydii and S. flexneri. Antibiotic susceptibility assay for 14 different antibiotics was carried out using agar disc diffusion method. Presence of ipaH gene was investigated by PCR using specific primers.
Results: From the results of this study the Shigella isolates were classified as follows: 36 (%73) Shigella sonnei, 9(%18) Shigella flexneri, 3(%5) Shigella boydii, 2(%4) Shigella dysenteriae. Approximately %50 of the Shigella isolates were resistant to Tetracycline and Cotrimoxazole. Shigella sonnei showed more resistance than other serotypes against the studied antibiotics. PCR assays showed that all isolates harbored ipaH gene.
Conclusion: The results showed that prevalence of Shigella sonnei is higher than other serotypes. The isolates showed high sensitivity to third generation cephalosporines and aminoglycosides. PCR detection of ipaH gene as a reliable marker for identification of Shigella species could be recommended.
Volume 14, Issue 1 (1-2011)
Abstract
Objective: Nowadays notable increase in acquired resistance of Candida species to antifungal drugs and necessity of using agents with antifungal properties is unavoidable. In some plants due to presence of components such as polyphenols have antimicrobial properties. In this study antifungal effects of essential oils of Thymus vulgaris, Petroselinum crispum, Cuminum cyminum and Bunium persicum on standard strain of Candida albicans were evaluated.
Materials and Methods: 25 grams leafs of the Thymus vulgaris and Petroselinum crispum and seeds of the Cuminum cyminum and Bunium persicum were dried and grinded after that the essential oils of each mentioned plant were prepared by Clevenger system. Serial dilutions of essential oils were made in 96 well microtiter plates. Minimum Inhibitory Concentration (MIC) and Inhibitory zone diameter were assessed by Microdilution broth and Disc diffusion agar techniques, MIC50, MIC90 and MFC were also determined.
Results: Minimum inhibitory concentration 90(MIC90) essential oils of Thymus vulgaris, Petroselinum crispum, Cuminum cyminum and Bunium persicum were respectively 25, 72, 412, 130 µg/ml and Minimal Fungicide Concentration (MFC) were 48, 146, 62, 280. Inhibitory zone diameters were 28, 20, 12, 15 millimetres.
Conclusion: In this evaluation essential oils of Thymus vulgaris, Petroselinum crispum, Cuminum cyminum and Bunium persicum showed suitable antifungal effects against growth of standard strain of Candida albicans. Thus these herbal essences after supplementary studies possibly can be suitable substitute for chemical medicine on Candida infections especially on mucocutaneous Candidiasis.
Volume 14, Issue 5 (9-2012)
Abstract
Pyramiding genes related to grain quality and resistance through marker assisted selection (MAS) is an important approach in rice breeding programs. Marker-assisted selection can be used for monitoring the presence or absence of these genes in breeding populations and can be combined with conventional breeding approaches. This study is a part of cultivar development program in Iran through integration of conventional breeding with marker assisted selection. Crosses between two high yielding transgenic lines carrying an insect resistance gene (cry1Ab, from Bacillus thuringiensis) with a local aromatic variety were made followed by selection for incorporation of insect resistance and aroma (fgr) genes in desirable single F2 plants. Finally, plants homozygous for aroma and carrying cry1Ab genes with good agronomic performance were identified. Further analyses are underway on these plants in F3 generation. These plants promise to develop new aromatic Bt rice lines through integration of classical and molecular breeding in the near future in Iran
Elham Sattari, Mojtaba Aghajani Delavar, Ehsan Fattahi, Korosh Sedighi,
Volume 14, Issue 10 (1-2015)
Abstract
In present paper the Inamuro Model based on free energy approach of the Lattice Boltzmann Method (LBM) was used to simulate the motion of bubble and coalescence of two bubbles under buoyancy force. By combining the Tanaka and Inamuro models, three-dimensional model of Inamuro was used in two-dimension for decreasing the computational cost. Firstly it was ensured that the surface tension effect and Laplace low for two density ratio 50 and 1000 were properly implemented. Secondly in next step, effect of governing dimensionless numbers problem such as Etvos number and Morton number on Reynolds number and terminal shape of bubble were investigated. Different flow patterns in various dimensionless numbers were obtained and by changing the dimensionless number, terminal change of bubble’s shape was seen. Finally, motion of two bubbles and terminal shape of coalescence of two bubbles were studied in different dimensionless number, which shape of first bubble was same to single bubble, but it was seen that second bubble experienced various shapes due to its location in wake of first bubble and less difference pressure on two sides of this bubble.
Ahmad Mohammadi Najafabadi, Iraj Sattarifar, Mohsen Rezaeian,
Volume 15, Issue 1 (3-2015)
Abstract
Cracks due to manufacturing processes or in-service applications can propagate and cause failure in structures. Therefore, it is of interest to find a suitable fracture assessment method for predicting crack initiation. Main approaches for fracture assessment of structures are global approach and local approach. In the global approach, it is assumed resistance against fracture can be measured by a critical values of a far from crack tip parameter like K or J. In this study, Beremin model of local approach is used for predicting brittle fracture which studies stress and strain fields at the crack tip. The model introduces unknown parameters which have to be calibrated using experimental fracture data. The purpose of this study is evaluating of conventional calibration methods of local approach parameters using the experimental brittle fracture data of three point bending specimens, determining limitations, and finally presenting a new calibration method to produce suitable parameters for predicting brittle fracture of the specimens by using local approach to fracture. This study shows that conventional calibration method using experimental fracture data of three point bending specimens has limitation in some cases. Also, by introducing location parameter of Weibull distribution as a stress triaxiality criteria in Beremin model, a new rational method for predicting brittle fracture of the three point bending specimens with different constraints is presented.