Search published articles


Showing 94 results for Soleimani


Volume 0, Issue 0 (Articles accepted at the time of publication 2024)
Abstract

The increasing ubiquity and impact of audiovisual content has turned it into one of the primary objects of study in a number of disciplines in humanities, including Translation Studies. In the recent decade, audiovisual translation (AVT) has been a thriving research focus in many parts of the world. This article aims at mapping AVT in the context of Iran by reviewing the published Persian AVT research worldwide. The article is organized into two parts: the first part provides an overview of the main research articles, delineating the main research trends in AVT research in Iran. The second part presents and discusses research gaps and areas that merit further scholarly attention by academia. The article concludes that research on the topic in Iran is still in a fairly early stage, with the studies focusing mainly on dubbing and subtitling of audiovisual products. Furthermore, areas such as accessibility and inclusion, in particular, voice-over and game localization are grossly under-researched. To fill the gap, certain areas are highlighted and recommended for future research.

Volume 0, Issue 0 (ARTICLES IN PRESS 2024)
Abstract

The present study investigates the effect of baking temperatures (140, 160, 180, 200, and 220℃) on texture kinetics. It also explores a statistical classification meta-algorithm, called Adaptive Boosting (AdaBoost), to predict texture changes during conventional cake baking. The experimental results indicated that texture properties were significantly affected by baking temperature and time. As time and temperature increased, there was an increase in hardness, cohesiveness, gumminess, and chewiness and a decrease in springiness. However, the impact of time and temperature on resilience was inconsistent, as it was maximum in the last quarter of the process. The predicted results revealed that the AdaBoost algorithm accurately predicted the texture properties with a high coefficient of determination (R2 > 0.989) and minimal root mean square error (RMSE < 0.0019) across all textural properties. Therefore, it can serve as an efficient tool for predicting the texture properties of cakes during baking. Furthermore, the proposed methodology can be extended to predict the texture properties of other baked goods.
 

Volume 1, Issue 1 (4-2014)
Abstract

Background: Differentiation ofmesenchymal stem cells (MSCs) to hepatocyte-like cells could be associated with development of liver function factors. The impact of differentiation-dependent changes on DNA integrity is not well understood. In this study, hepatocytes and their progenitor stem cells were treated with aflatoxin B1 (AFB1) and amplification of selected genes linked to DNA damage was examined. Methods: MSCs and CD34+ cells isolated from umbilical cord blood (UCB) were treated with AFB1 (0, 2.5, 10 and 20 µM) in selective media supporting the hepatocyte differentiation. After 24 htreatment the DNA damage (Comet assay) and amplification rates ofP53 and β-globin genes were measured using real time polymerase chain reaction (QPCR). Results:The results show that AFB1 treatments resulted in a concentration- dependent increase in the DNA damage and suppression of the specific gene amplification. The extent of DNA damage was significantly greater in hepatocytes differentiated from MSCs when compared to those obtained from CD34+ cells. The effects of AFB1 on the rate of selected gene amplification in QPCR showed that the lesions (expressed as lesions/10 kb) in P53 and β-globin genes was significantly greater in hepatocytes derived from MSCs as compared to the cells derived from CD34+ cells. Conclusions: These data together with the results of cytochrome P450 (CYP3A4) expression in the cells suggest that the non-differentiated stem cells are probably less vulnerable to genotoxic agents as compared to hepatocytes differentiated from them.

Volume 1, Issue 2 (7-2014)
Abstract

Background: In recent years we have successfully adopted an in vitro hepatogenic differentiation of mesenchymal stem cells (MSCs). In this protocol the biologically active hepatocyte-like cells were differentiated from the stem cells isolated from either bone marrow or umbilical cord blood (UCB) samples. The aim of the present study was to compare the efficiency of the hepatogenic differentiation of MSCs isolated from UCB and MSCs. Methods: Differentiation process of MSCs was carried out in a selective medium supporting hepatogenic differentiation for 3 weeks. Then using specific markers we have examined the hepatocyte formation following hepatogenic differentiation of the stem cells. Hepatogenic markers namely albumin, α-fetoprotein (AFP) and cytochrome P450 3A4 (CYP3A4) were monitored at different time intervals during differentiation. Results: Transdifferentiation of the UCB and bone marrow MSCs was also characterized by measuring albumin, AFP and CYP3A4 at mRNA levels using reverse transcription polymerase chain reaction (RT-PCR). AFP was expressed in the undifferentiated UCB-MSCs and increased on day 21 of differentiation. However, AFP was not detected in the undifferentiated bone marrow MSCs. But, AFP expression started during the first week of differentiation. Albumin expression was detected in hepatocytes from UCB as well as bone marrow. The expression of albumin and its secretion from hepatocyte prepared from bone marrow appeared earlier compared to the cells derived from UCB. Metabolic function of the hepatocytes evaluated by secretion of albumin in the culture media was also similar in the cells isolated from both the sources. Conclusions: The differentiation potential of MSCs derived from human UCB and bone marrow under in vitro condition is comparable. However, it appears that there is time-dependent difference in the onset of expression of liver specific markers particularly albumin synthesis in hepatocytes derived from different stem cells.

Volume 1, Issue 2 (Fall & Winter 2025)
Abstract

Seyyed Jamaluddin Asadabadi is one of the reformers of religious thought in the contemporary world, who had an impact not only in Iran and among Shiite Muslims, but also throughout the Islamic world, including in Afghanistan, Egypt, Turkey (Ottoman) and Iraq. He wanted to save Muslims from colonial rule and the backwardness of the world and achieve the greatness of Muslims. The main question of the current research is following the issue of whether adopting a strategy and opening up ideas in Egypt's affairs could create a confrontation in its relations with the colonial countries? And whether the adoption of this strategy in those periods of time was in accordance with the national interests and the domestic and international conditions of Egypt? The result of the research, which was conducted with a descriptive-analytical approach, indicates that Seyyed Jamaluddin's intellectual capacity has influenced a wide scale of the Islamic world. Therefore, in order to achieve this goal, Sayyid suggested the way to unify the Islamic world and return to authentic Islam.

Volume 2, Issue 1 (Winter 2022)
Abstract

Challenging the entire western philosophical tradition, which in his opinion has caused useless theoretical dualisms throughout the history of philosophy, Rorty wants to attempt to deconstruct and eliminate these dualisms in the context of "redemptive literary culture". By creating a dividing line between the private and public spheres, Rorty wants to specify the contribution and involvement of philosophers in presenting theoretical and philosophical views and to say that the political sphere does not need to acquire foundations from the individual criteria of the private sphere. It is as if Rorty wants to prevent the philosopher's ambitions and interference with theorizing by reducing philosophy to literature. In fact, he believes in the distinction between private and public spheres or politics, the philosopher's tool is imagination and his intellectual sphere is literary culture and his place is the private sphere. Assuming the acceptance of pragmatic criteria, doesn't this division of a person in two completely different areas make him a dual personality? Can this intellectual stance be reasonable and acceptable?


Volume 2, Issue 2 (Spring 2022)
Abstract

Criticizing the history of philosophy and epistemology definitely requires great courage, because the effects and consequences of this can lead to dangerous results. When we ignore any metaphysical and superhuman beings and generally explain any epistemological issues in the explanation of our knowledge strategy, then the result can lead to nihilism, absurdism, disorder and anarchism. It can be dangerous and toxic both for the individual and for the society. While we expect to encounter such nihilistic thought in Rorty's thought, on the contrary, the philosopher in question claims that his thought will not be led to what was mentioned. Rather, it seeks pleasure, happiness and happiness for humans, because although there is no longer talk of truth and goodness, but by expanding the solidarity between human "us" as much as possible, it seeks to change this road and move towards a human utopia. Our purpose of writing this article is to investigate and analyze the position of the epistemological foundations of ethics in Rorty's thought.



Volume 3, Issue 1 (6-2014)
Abstract

The effect of six diets including Scenedesmus quadricauda), manure (cattle+poultry, 1:1 ratio), vegetable (spinach+parsely+coriander, 1:1:1 ratio), algae+soil, vegetable+soil, and manure+soil were examined on culture of freshwater copepod, Acanthocyclopsrobustus, under the experimental conditions of 23±1ºC, 12 hours dark: 12 hours light photoperiod, and light intensity of 60 µmol photons/m2/s. The maximum population density (1282.6±163.7 individuals/L), maximum specific growth rate (SGR) (0.17±0.0 /day) and minimum doubling time (Dt) (4.0±0.1 days) were obtained when A. robustus fed on algae+soil. The nauplii production was 727.3±42.4 (±SE),  272.0±23.5, 267.6±17.7, 147.0±18.2, 33.6±3.8 and 25.0±7.1 ind./L and copepodit production was 311.3±26.8, 124.3±11.1, 183.0±13.0, 59.0±7.1, 14.3±8.1, and 17.6±3.6 ind./L in algae, algae+soil, vegetable, vegetable+soil, manure+soil, and manure, respectively. Correspondingly, the adult production was 208.5±26.8, 51.6±16.5, 192.7±23.7, 91.7±28.4, 8.7±5.4 and 8.7±3.6 ind./L, respectively. The maximum body length (663.8±29.1 µm) and width (526.2±23.7 µm) of A. robustus were recorded when fed with vegetable+soil and vegetable, respectively. The number of nauplii, copepodit, adults, length and width were significantly correlated with EC, BOD and COD. According to results, the A. robustus has suitable potential culture on algal and non-algal diets based on production, growth, body size, BOD and COD, but the better performance obtain on algae+soil and vegetable.

Volume 3, Issue 2 (Spring 2023)
Abstract

Neo-Fregean metaontology is one of the most controversial metaontologies in recent decades. Regarding this metaontology as a deflationary metaontology such as quantifier-variance or maximalism prevails nowadays. But, if we consider Frege's views and the consequent metaontology, it will be obvious that there isn’t any relation between Fregean metaontology and deflationary metaontologies. Hence, interpreting neo-Fregean metaontology in the light of Fregean metaontology categorizes it in the class of serious metaontologies. In this reading, the crucial point is that the metaontological language is the logical core of natural language rather than mere natural language. Notwithstanding that this reading is consistent, it has implications for neo-Fregean methodology, namely, that neo-Fregean metaontology's function is metaphysical - in the determination of the category of abstract objects - and epistemological - in justification of our knowledge about these objects – but not ontological.
 

Volume 4, Issue 1 (4-2014)
Abstract

Paying little attention to the identity of Iranian architecture is one of the problems of contemporary architecture of Iran which has caused the absence of a precise definition of Identifying criteria in contemporary architecture of Iran; so that diversity of selected views is one of the main features of this architecture. Perhaps it can be said that many Iranian architects design without codified principles and even without theoretical knowledge and precise understanding of mental concepts in different styles. However, the lack of attention to values of Iranian traditional architecture is followed by eclecticism crisis in contemporary architecture. The questions raised in this context is whether a new definition of architecture can be given by examining the thoughts and opinions of Iranian philosophers like Mulla Sadra gave a new definition of architecture and whether according to their theories outlined principles Can be codified for contemporary architecture? The general approach in this research which intends to paraphrase the ideas of Mulla Sadra in contemporary architecture is Interpretative - historical and descriptive. The research data are derived mainly through observation and documentary studies. The results indicate that by using Mulla Sadra's definitions of being (al-wujud) and essence (mahiyyat) we can have a new defined view at architecture. As Sadra considers all phenomena in two dimensions, architecture also has both being and essence aspects. The essence of architecture responds to material needs and its being is a truth which architecture maintains and brings it to presence. Indeed, the Physics of architecture is the essence of architecture (mahiyyat) and the valuable concepts underlying the architectural Physics are the being of architecture (al-wujud). Considering Mulla Sadra's primary of being (asalat al-wujud) we can conclude that there is also primary of being in architecture. Of course this does not mean that the architect who cares about the being of architecture pays no attention to the essence of architecture. It means that the architect who cares about the being of architecture tries to discover and understand the being of traditional architecture and to present it in a new body which is appropriate for today's architecture. Therefore by regarding the Principles of Mulla Sadra's thought and practice used to achieve the roots of his philosophy, principles can be formulated for architectural design. The principles are formed in order to achieve the lofty goal of traditional Iranian architecture and they attend guide and direct the audience attention toward the pure being (God). Using concepts as inspiration and metaphor and manifestation of the light can be useful to achieve this goal. These concepts are the essential to make the basis clear for contemporary architecture and shows that we can achieve our goals for contemporary architecture of Iran in the same way that Mulla Sadra was able to resurrect the underlying concepts of traditional philosophy. From the perspective of al- wujud ontology, the first target of architecture is to show the truth of being essences; to show the being within the essences without them there is no sense of architecture. If we consider the context of architecture as an activity consisting of a combination of emotion and symbolic meanings, according to Mulla Sadra's method, architecture must accommodate itself to life as a significant whole. In this case the main task for architects will be to shape living spaces and to create necessary content for human presence (al-hozur) and to provide features for human identification. So there is no doubt that one of the main goals for architecture is to create the perfect place and to promote the human presence (al-hozur). Architecture is the genuine sense of human presence (al-hozur), and it may be understood by the human presence (al-hozur). Creation of art is a kind of creation that its nature is extracted from god. According to Mulla Sadra's principles, man unites with his artwork and then the truth would emerge. Architecture is a part of the whole art that tends to reveal the being (al-wojud). From the perspective of being and essence (al-mahiyyat) architecture itself is consisted from two sides; one side is being and the other is essence. And the perception of its essence is possible because of its being: but the being of architecture obtains its existence from the creator and so architecture is always in compliance with its creator's rank of being (mrateb al-wojud). In this theory the origin of architecture is the being of architecture (al-wojud) and the essence of architecture (al-mahiyyat) is manifested through its being. If the architect is higher in the rank of being his designs will also be higher and more spiritual. Therefore as mentioned, there is no doubt that one of the main goals for architecture is to create the perfect place and to promote the human presence (al-hozur). To improve the rank of human being by the effect of space is among the most sublime objectives of architecture. According to al-wojud Philosophy and Mulla Sadra, when an architect designs the different parts and details of a building it is necessary to emphasize the overall structure of notion and so the overall structure of space and of the geometry which is known as the architecture generator. The two mentioned elements are such abstractions in architecture and to emphasize them is to help us accept the concepts and principles of the being (al-wojud) for the prospective architecture considering the variables that influence it. This structure is basically a coded architecture. Since al-hikmat al-mota'alie is based on alhikmat al-eshragh and in alhikmat al-eshragh god or al-wajeb al-wojud is equal to the absolute light, therefore the light manifested in architecture, is an allegory of al-wajeb al-wojud or god. The sublime architecture upgrades man to the level of meaning and wisdom and leads him toward the perception of al-wajeb al-wojud. Architecture answers to this character of perception by metaphor. According to Sadra's point of view Subscription between the human spirit and architecture is required so that the presence of pure meaning in human perception is based on the human presence (al-hozur) itself.

Volume 4, Issue 2 (Spring 2018)
Abstract

Aims: Nowadays, treatment of bacterial infections is one of the most important challenges in the world. Medicinal plants offer a great hope to overcome these needs because of their chemical diversity and their significant role in the drug development. The aim of this study was to evaluate the in vitro antibacterial activity of the thyme (Thymus vulgaris) essential oil against Mycobacterium tuberculosis.
Materials and Methods: In this experimental study, thyme herb plants were collected and thyme essential oil was extracted. The Minimum Inhibitory Concentration (MICs) tests were performed to determine the antimicrobial activity of Thymus plant against the first (Isoniazid, Rifampicin, Ethambutol) and second (Cycloserine, Streptomycin, Kanamycin) drug antibiotics of mycobacterium. Data were analyzed by SPSS 21 software, using one-way ANOVA test.
Findings: The MICs for Isoniazid, Ethambutol, Streptomycin and Cycloserine were less than 10µg/ml and the MIC values for Rifampicin and Kanamycin were 40µg/ml. The limits of minimal inhibitory concentration of essential oil was between 0.5-40µg/ml (p<0.05).
Conclusion: Thyme essential oil has antibacterial activity against Mycobacterium tuberclusis.

Volume 4, Issue 4 (Fall 2024)
Abstract

Regarding the characteristics of myth in various mythological approaches, it is evident that myth functions in the culture, society, mind, or psyche of human beings, has a collective aspect, is connected to an unconscious or preconscious state, and finally has a normative dimension. These characteristics make social ontology an appropriate place for the analysis of myths. Following the ideas of some social ontologists such as Searl or Tuomela, myth can be considered an institutional fact. However, according to the dominant approach in social ontology in the contemporary analytic tradition, all institutional facts are implicated by individual or collective intentionality. We argue that the peculiarity of myth’s connection with the unconscious state challenges all subcategories of the dominant approach in contemporary social ontology; hence, a shortcoming of the dominant approaches in analyzing myth as an intuitional fact.
 

Volume 5, Issue 3 (12-2016)
Abstract

 Fish protein hydrolysates from whole kilka, using alcalase enzyme (ratio 1: 100) under optimal temperature (55°C) and pH (8.5) was evaluated for its hydrolysis degree and antioxidant activity. Results of the hydrolysis degree recorded at time intervals of 1, 2, 3 and 4 hours indicated the hydrolysis degree increased with increase in the hydrolysis time. The evaluation of FPH antioxidant activity, using DPPH, ABTS and reducing power assay tests at 3 concentrations (1, 2 and 5 mg/ml indicated the highest inhibitory effect at 5 mg/ml was 74.4%, 72.3% and 1.8 absorbance in 700 nm for DPPH, ABTS and reducing power assay, respectively. Generally, the findings of this research confirmed the potential of kilka as a source of natural antioxidants for food applications.

Volume 5, Issue 4 (winter 2024)
Abstract

Aims: Despite the existence of potential human and executive forces in Iran, industrialization and the use of materials in buildings in order to prevent the waste of materials, has gone through a very slow process, and considering the importance of the construction sector in Iran's economic activities, it is necessary to take effective measures to be done in this context. For this purpose, this research has been carried out with the aim of identifying and ranking the effective indicators on the waste of materials according to the existing theoretical foundations.
Methods: In terms of the purpose of this research, it is a developmental type of research, and in terms of the method of doing the work, it is descriptive, survey and document type. In order to identify the effective factors, a sample was selected from among the community of experts in this industry with a simple random sampling method, and the desired data was collected by distributing a questionnaire and analyzed with SPSS statistical software.
Findings: The research showed that proper design  has the greatest effect on the increase in construction waste. After that, the construction industry  in the second place, education  in the third place, financial resources and economic justification  in the fourth place and finally the reserve and warehouses  have the least impact on the increase of waste.
Key Words: Modern Materials, High-Rise Buildings in urban centers, Zero Waste.
 

Volume 6, Issue 2 (9-2016)
Abstract

The present study was conducted the effect of internal marketing on critical thinking according to mediator management styles. The research is implemented by descriptive - survey method and its statistic society is consisted of staff and general managers of hotels of Isfahan which between them using random - stratified sampling, 172 questionnaires were received and evaluated. To gather information from the questionnaires in this research internal marketing questionnaire constructed by author and critical thinking questionnaire of Vitli have been used that Its validity is verifiable and has been evaluated by professors and Cronbach's alpha was used to assess reliability. Obtained alpha for internal marketing is 0.87 and for critical thinking is 0.88 and for management styles is 0.90.To data analysis statistical methods of Amos correlation coefficient and SPSS software of data by Spearman software and regression analysis and structural equations were used. The results confirmed the presented conceptual model and show that there is a relationship between internal marketing and critical thinking of employees and also show that internal marketing have significant and positive effect on critical thinking with regard to management style.

Volume 7, Issue 2 (No.2 (Tome 30), (Articles in Persian) 2016)
Abstract

The purpose of this research is studying the structure of tense and aspect in Bala-Guerive Luri dialect. Bala-Guerive Luri is a variety of Luri dialect spoken by the inhabitants of Khorram Abad city, Lurestan province, Iran. The data have been collected through a ten hours recording of everyday conversations of Lur informants; and analyzed descriptively. This dialect has two tenses: past and non-past. Thus there are simple present, simple past, present perfect and past perfect. The representation of aspect in this dialect is different from Farsi. In this dialect perfective aspect is represented by simple past, present perfect and past perfect. Imperfective and progressive aspects in present time are shown by /h/ and in the past time are shown by /he: /. imperfective aspect is also shown by present perfect structure. Proximate aspect in the present time is shown by/ h/ with agreement to main verb in person and number and in the past time is shown by / hs/ with no agreement to main verb.

Volume 8, Issue 2 (No. 8 (Tome 37), (Articles in Persian) 2017)
Abstract

Working Memory Capacity and Learning L2 Structures under Explicit and Implicit Condition: The Effect of Type of Linguistic Structures
Working memory capacity (WMC) is shown to be correlated with explicit learning conditions, but not implicit (Reber, Walkenfeld, & Hernstadt, 1991). However, recent study shows that the relationship is more complicated (Tagarelli, Borges-Mota, &Rebuschat, 2015) and some other factors such as linguistic complexity can mediate the relationship between WMC and learning condition. This study investigated the relationship between WMC and acquisition of two English linguistic structures with varying degree of complexity, namely past simple tense and relative clauses under explicit and implicit learning conditions. Fifty-nine intermediate Persian learners of English took part in this study receiving either explicit or implicit instruction on two grammatical structures defined as easy (simple past tense) and difficult (relatives) at classroom setting.  Their ability to use these structures was measured at three times; once before treatment and twice after it through a timed grammaticality judgment task and an elicited oral imitation task. Complex listening span task was used to measure their working memory capacity. The results of the two-way repeated ANOVA revealed that explicit and implicit instruction were significantly effective in development of easy and difficult structures. The results of correlation for working memory capacity and learning condition revealed that there is no significant relationship between WMC and learning under implicit condition. This result hold true for both simple and difficult linguistic structures. However, under explicit condition, differential results were found for different linguistic structures. Whereas no relationship was found for simple linguistic structures, the relationship between WMC and learning difficult linguistic structures under explicit condition was found to be significant.

Volume 9, Issue 1 (No. 1 (Tome 43), (Articles in Persian) 2018)
Abstract

 “Verbal versus adjectival” dichotomy of passive structures has occurred in studies based on approaches believing two generative component in the grammar, i.e. syntax and lexicon. Distributed morphology (DM) (Halle & Marantz, 1993), a non-lexicalist approach to morphology, takes the theoretical position that there is only one generative component in the Grammar, the syntax, and claims that all derivation of complex objects is syntactic. This descriptive-analytic article is aimed to study the so-called passive structures and the participles used in them, in Persian within the framework of DM, from the perspective of syntax-semantics interface, focusing on Embick (2004). The article tries to answer the following two questions:
 a. How can we, based on DM, give a unified analysis of passive     structures in Persian and so end the arguments among linguists regarding the existence of passive in Persian.
 b. What syntactic features within DM, are responsible for some Persian participles having different interpretations (eventive and resultative).
Regarding the first question, it is predicted that, since Persian verbal and adjectival passives are all complex objects, their derivations, based on the present approach occur in syntax. Regarding the second question, it is hypothesized that, since some Persian participles such as godɑxte, bæste and ɑrɑste, in “participle + ʃodæn” structures in passive structures, have different interpretations, there is another kind of participle (resultative) in Persian.
After analyzing the data, it is argued that since Persian verbal and adjectival passives are all complex objects, their derivation, based on the present approach and contrary to previous studies, occur in syntax and their distinction is attributed to the position at which aspect head is merged (above or under v). In other words in verbal (eventive) passives the aspect head is merged above “v head”, while in simple adjectival passives it is merged under “v head”.
Also based on linguistic and interpretive evidences, findings showed that some participles (godɑxte, bæste and ɑrɑste) in “participle + ʃodæn” structures are interpreted as both event and resultative. So another kind of participle (resultative), in Persian will be introduced and its unique syntactic features in DM framework will be specified. The significant syntactic difference between the eventive and resultative passives is first attributed to the feature [AG] which is present in eventive passive and missing in resultative ones, and second to the different uses of “ʃodæn” : as auxiliary in verbal passives and copula or BECOME-operator (Embick, 2004) in resultative passives.
 
Keywords: Distributed morphology; Underspecification; Lexicalist participle. 
 

Volume 9, Issue 1 (Winter 2018)
Abstract

Aims: The induction of artificial over-expression of miRNAs is an appropriate approach to more effective cell differentiation. The significant role of microRNA-1(miR-1) has been reported in the development and differentiation of cardiac cells. Lentivirus is an effective vector for stable cell line production. The aim of this study was the production of recombinant HEK293T with miR-1 overexpression as a biological model for cardiac studies.
Materials & Methods: In this experimental study, HEK 293T cells were cultured in DMEM medium with 10% Fetal Bovine Serum (FBS) and L-glutamine 2mM and Penicillin-Streptomycin 1X in incubator medium. After cloning of miR-1 gene, recombinant clones were selected and the recombination was confirmed by sequencing. The miR-1 carrying vector and auxiliary vectors were packaged in the HEK293T to produce the recombinant virus. The infection of HEK293T by recombinant virus was performed in order to achieve stable cell line. Then, GFP fluorescent marker evaluated the efficiency of transfection and effective virus dilution. Finally, the alteration in expression level of miR-1 was assessed by qPCR. Data analysis was performed by comparing the threshold cycle and Pfaffl method.
Findings: The most GFP expression was detected in transfected cells by 150 micromole dilution. GFP fluorescent marker facilitated optimization and purification of recombinant cells. qPCR investigation demonstrated the significant increase in expression of miR-1 in transfected cells in comparison to controls.
Conclusion: The stable recombinant HEK293T miR-1 over-expressing cell line in lentivirus can be utilized as a suitable biological model for investigation of cardiac evolution and development processes.

Volume 9, Issue 1 (Winter 2018)
Abstract

Aims: Marine macroalgae are diverse organisms with adaptation for live in stressful environments. The aim of this study was to investigate the biological activities of organic extract; n-Hexane (nH), ethylacetate (E) and methanol (M) of three green alga from family Ulvaceae, Ulva clathrata, Ulva linza and Ulva intestinalis, collected from the coast of Bandar Abbas.
Materials & Methodes: In this experimental study, for identification the superior species, the tested activities included antioxidant assay at gradient concentrations by ferric reducing power assay, total antioxidant capacity, total phenolic content, and brine shrimp cytotoxicity activity of these extracts on model organism, Artemia salina. Data analysis was performed by one-way analysis of variance and Duncan's multiple tests at 5% probability level using SPSS 21 software and drawing charts using Excel 2013 software.
Finding: The more effective algal extracts by maximum antioxidant capacity, were recorded for M extracts of U.intestinalis, E and M extracts of U.linza and U.clathrata. The algal extract exhibited a higher antioxidant activity in comparing to ascorbic acid (as a standard) with significant differences between the extract in different concentrations (p≤0.05). The result showed the highest content of total phenol were recorded for the M extracts of U.linza and U.clathrata which confirmed the findings of other researchers that the increase in free radical scavenging activity of natural extracts is associated with the content of phenolic compounds. The highest brine shrimp cytotoxicity activity was recorded for the nH extracts of U. linza (LC50= 300.78 mg/ml). According to the results, in general, U.linza can be introduced as a priority species for biological properties and in further studies.
Conclusion: Three green alga from family Ulvaceae, Ulva clathrata, Ulva linza and Ulva intestinalis, have antioxidant and cytotoxic activity. U.linza due to the high amount of phenol and high antioxidant power can be introduced as a priority species for biological properties.

Page 1 from 5    
First
Previous
1