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Projected global climate change may have a major influence on crop yield. The likely effects of climate change caused by increasing atmospheric carbon dioxide levels on rice yield in Iran were evaluated using a mechanistic growth model for rice, GSAC-rice, running under a climate change scenario predicted for a doubled-CO2 (2xCO2) atmosphere by the Geophysical Fluid Dynamics Laboratory (GFDL) General Circulation Model (GCM). Simulations were run for two locations with contrasting climates, one in the north (Rasht) and one in the south (Ahwaz) of Iran. GFDL predicted that as a result of doubling CO2 , temperature increases by 4.5 and 4.6 0C during the rice growing season in Rasht and Ahwaz, respectively. Changes in solar radiation are minor, but rainfall during the rice growing season decreases by 38.8% (102 mm) for Rasht and 68.2% (5.8 mm) for Ahwaz. It was predicted that doubling [CO2] alone increased rice yield by 30%, but that yield decreases by 3.7 and 11.6% for each degree centigrade rise in temperature in Rasht and Ahwaz, respectively. As a result of the combined effect of both doubling [CO2] and the climate change accompanying it (predicted with GFDL), 8% greater rice could be produced in Rasht, but irrigation needs would be increased dramatically by 57%. In Ahwaz (the south of Iran), rice production could be halved and might not even remain a viable option unless plant breeders are able to produce more heat tolerant rice cultivars. It was concluded that rice production in the north and south of the country would change dramatically.

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SARAY" is part of the intangible heritage of the Azerbaijani people rooted in the culture, mentality and attitude of the people. So, it has been represented in oral literature, music, stories and poetry. In this study, the intangible cultural heritage is considered a cultural property, which pave the way to represent will, resistance and individual arguments of actual community activists to pass the crisis social and cultural contexts. The conceptual representation belongs to notion of Plato emerging in the idea and practice of recent intellectuals in the field of spiritual heritage which is now the main academic subject. This study was conducted qualitatively and has a descriptive and phenomenological research methodology. The purpose of this study is to investigate the permanent character of spiritual values or their representation in the urban life of the inhabitants of Ardebil by studying the spiritual heritage of Saray in the modern life of women in Ardabil. Intangible cultural heritage contains the historical, cultural, spiritual, scientific, and aesthetic values of current era, and can contribute to the growth and mobility of shared values and representations of values in everyday life. On the other hand, it refers to the existence of the culture of resistance and ethics of the region, and today it can be represented in the renewal of moral values, the commitment to the family for the present generations and future.

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Phytophthora root and crown rot of soybean is known as a destructive disease of soy-bean both through out the world and in Iran. Physiologic races of Phytophthora soja were determined in this research and also a fast, accurate and simple method for inoculation of soybean to test race specific resistance. During the years　2001-4, infected soybean plants at different growth stages were collected from different areas and 22 isolates of P. sojae were recovered using PARPH medium. Physiological races of the pathogen were deter-mined on differential seedling lines by the hypocotyl inoculation method. Ten seedlings from each differential line grown in a 10-cm pot were inoculated under greenhouse condi-tions (25ºC) by a 10-14 days old fungus (LBA medium). The reaction of the seedlings was classified after 5-6 days as resistant (70% or more of seedlings alive) or susceptible (70% or more of the seedlings killed). Most isolates were identified as race one, six as race three, one as race four and one as a putative race 13. Race-specific resistance of the 60 cultivars towards race three was determined. Some of them such as ‘TMS’, ‘Maverick and ‘Williams 82’ were considered as resistant cultivars. All experiments were repeated three times.

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Objective: β-thalassemia is caused by absence or reduction of β-globin chain synthesis. One of the effective therapeutic methods for this disease can be gene therapy by viral vectors. The capacity of lentiviral vectors is approximately 8 kb, we designed a 6 kb construct containing mini LCR and β-globin gene instead of LCR region. The aim of this study is to make a recombinant lentiviruses containing miniLCR and β-globin gene for transfer to the target cells for gene therapy of β-thalassemia. Materials and Methods: HS2, HS3, HS4 segments (mini LCR) and β-globin gene with 5΄ and 3΄ UTR were amplified from the genomic DNA of a normal individual by PCR. Each segment was cloned in pTZ57R/T vector and then sub cloned first into the pBGGT vector and finally into the pLenti-Dest vector. Final transfer vector and the three helper packaging plasmids (Plp1, Plp2, Plp/VSVG) were cotransfected into 293T packaging cells using lipofectamine 2000. Harvested viruses were confirmed by RT-PCR on extracted RNA of these recombinant lentiviruses. Results: The titer of lentiviral stock determined in a K562 cell line and compared with COS-7 cell line. The titer in both cell lines was the same. Optimum MOI for COS-7 cell line was 5 and when polybrene was used transduction increased by 2 fold. The remaining transduced COS-7 colonies were expanded and DNA was extracted. By PCR, random integration of construct into the genome was evaluated. Conclusion: The produced lentiviruses can be an appropriate means for effective transfer of the designed construct into dividing and non-dividing cells such as hematopoetic stem cells for transplantation of beta thalassemia patients. Efficiency of transduction by leniviruses is more than the gene targeting technique. Also units of HS2, HS3 and HS4 regions in mini LCR and selection of larger HS3 unit may increase the expression of beta globin gene.

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Objective: Alpha-thalassemia is one of the most prevalent hemoglobin disorders in the world and it is a common hereditary condition caused by deletion of one or more α-globin genes. Common α-thalassemia deletions like 3.7 kb, 4.2 kb, 20.5 kb and Med can be detected by Multiplex PCR. There are, however, some unknown deletions that can not be detected by the mentioned method or even by direct DNA sequencing. In the present study, Real-time PCR was used to determine the presence or absence of unknown deletions. Materials and Methods: Real-time PCR was performed using intercalating dye SYBR Green I and α1, α2 and CLCN7 genes were amplified. Data analysis was conducted using comparative threshold method (ΔΔCT) for determination of Gene dosage of α1-globin and α2-globin genes. Results: The results showed the ratio of 0.90±0.16 for normal individuals and the ratio of 0.32±0.15 for carrier samples with deletions. In addition, Melting curve analysis confirmed the specific amplification of target genes. Conclusion: The Real-time PCR assay is simple, rapid, and reliable. It can be applied for direct determination of unknown deletions in Alpha-thalassemia carriers.

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ABSTRACT Rosa damascena has attracted considerable attention in horticulture, biochemistry and in pharmacology because of the fragrance of the flowers and the high content of its bio-logically active substances. There is limited information available on factors controlling flower yield and its components. The present study reports an investigation on flower yield and the various factors affecting it, in Iranian Rosa damascena using sixteen geno-types collected from different regions in Iran. These genotypes were planted at Isfahan Agriculture Research Center, using a randomized complete block design with three repli-cations in 2005. Twelve yield determining characters including flower yield per plant, number of flowers per plant, fresh flower weight, plant height, canopy diameter, length and width of bud, number of petal per flower, length and width of receptacle, fresh weight of petal as well as flowering period were recorded. Rosa damascena genotypes sig-nificantly varied for all the traits except for the fresh weight of petals per flower. Pheno-typic and genotypic coefficients of variation for flower yield per plant (48.03%, 36.49%), number of flowers per plant (40.65%, 26.99%), number of petals per flower (37.56%, 32.31%) were higher than the coefficients for other tested traits. Cluster analysis revealed that Khuzestan and Shiraz 2 genotypes were the most related ones, while the most inde-pendent ones were the western and eastern Azerbaijan genotypes. Results of stepwise re-gression analysis showed that 90 percent of total variation of flower yield per plant could be explained by the number of flowers per plant. This suggested that number of flowers per plant is the most important component determining flower yield per plant. According to principle component analysis, parents with a high standing of the following traits: fresh weight of flower, number of petals per flower and bud width, may well be employed for hybridization in a breeding program. Regression and cluster analyses proved to be the appropriate multivariate analyses for an identification of Rosa damascena genotypes pos-sessing the desirable characters for hybridization to develop improved cultivars.

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In the current era, innovation and innovation performance are inevitable in many organizations. Decentralization creates flexibility that enables companies to respond quickly to changing market, customer and technology needs. Employee involvement creates dynamism in the organization and absorptive capacity as an important indicator enables companies to acquire, absorb, transform, and exploit knowledge to maintain competitive advantage. Therefore, the purpose of this research is to examine organizational aspects such as decentralization, employee involvement, absorptive capacity and their interactions on the performance of business innovation and business performance. The research method was survey-analytical and using a questionnaire. The reliability of the questionnaire was confirmed using Cronbach's alpha coefficient which was 0.943 and the validity was confirmed based on the content validity measurement and Lavche formula (CVR). The statistical population includes the staff of the General Department of Wagons. Using random sampling, 50 questionnaires were completed and analyzed by SPSS software. The results of the research show that decentralization, employee involvement and absorptive capacity are positively associated with innovation performance and innovation performance also affects business performance.

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Objective: Sea cucumber is a traditional food and medical item and has been reported to exhibit Antioxidant, antifungal, anti tumoral and antibacterial bioactivity. The objective of this study is to describe the antibacterial activity of 4 extracts of Holoturia. Sp (sea cucumber), collected from Hengam Island of Persian Gulf. Materials and Methods: Methanol, hexane, aqueous and chloroform extracts from body wall tissue of the sea cucumber were screened for antibacterial activity against three strains of Escherichia coli Top 10 F´, TG1 and K12 using disc diffusion and broth microdilution tests methods. Result: The growth of all these strains were inhibited using concentration from 0.78 to 100 mg/ml of methanol, hexan and chloroform extracts. Among the extracts just methanol and chloroform with 100 mg/ml had bactericidal effect on TG1 and K12 strains. On the other hand, Aqueous extract had induced growth in of the all strains. Conclusion: The results suggest the possibility of applying sea cucumber as source of potential anti bacterial agents, whose compounds can be good candidates to make antibiotic products.

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Objective: Hearing loss is the most frequent neurosensory defect in human. Mutations in GJB2 and GJB6 are responsible for 50% of autosomal recessive non-syndromic hearing loss (ARNSHL) cases. Here we report on the frequencies of GJB2 and GJB6 mutations and three large deletions spanning the GJB6 gene including Del (GJB6-D13S1830), Del (GJB6-D13S1854) and a >920 kb deletion in patients affected by ARNSHL referred to Kawsar's Human Genetics Research Center. Materials and Methods: In this study, 94 patients from 63 families with ARNSHL were investigated. Patient's homozygote for 35delG were screened and left out of the study and the remaining samples were analyzed by sequencing of GJB2 and GJB6 genes. Also the three large deletions spanning the GJB6 gene were analyzed by Real Time PCR Results: In this study we found GJB2 mutations in 13 families (20.6%) out of 63. The 35delG mutation was the most common mutation in the studied population (61.5%). Other GJB2 mutations were delE120, R127H, W24X, and V37I. The heterozygous or negative cases for the GJB2 mutations were screened for mutation in the GJB6 gene by sequencing and no mutation was observed. Also, we checked the three large deletions in GJB6, we found no mutations. Conclusion: Low frequency of mutations in the GJB2 gene implies that other genes may be involved in causing non-syndromic hearing loss in our country.

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The short arm of rye (Secale cereale L.) chromosome 1 (1RS), besides being part of the rye genome, is present in many hundred wheat cultivars as either 1RS.1BL or 1RS.1DL wheat-rye translocation. In this study, the distribution of the wheat–rye translocation was examined in 33 Iranian winter and spring wheat cultivars, nine of which had a known donor of 1RS.1BL translocation and the other 24 were randomly selected cultivars without a known source of 1RS.1BL in their pedigree. The presence of the translocation was verified in 4 cultivars, using genomic in situ hybridization analysis. We also compared the Na+ exclusion and K+/Na+ ratios in leaf and root of the identified 1RS.1BL translocations and in eight randomly selected non-translocated (NT) control cultivars grown in hydroponic solutions, containing 0 and 200 mM NaCl. Mean comparisons showed that the 1RS.1BL cultivars (Atrak, Dez, Falat, Rasul) had significantly lower rates of whole plant dry weight and root dry weight in the presence of 200 mM NaCl compared with NT control cultivars. No significant difference was observed between translocated and NT cultivars for Na+ concentrations and K+/Na+ ratios in their leaves or roots. Although, there are many useful genes in 1RS arm, it has no substantial contribution to Na+ exclusion in comparison with NT controls at seedling stage.

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Objective: Traditional methods for storing microbial DNA samples use the low-temperatures of 4 °C, -20°C or -80 °C. The DBC Card is a new method of storing microbial DNA at room temperature. This study evaluates the stability and determine the best storing method of Bacterial DNA on the DBC card. Methods: In this study, we used a pair of primers from the conserved domain of 16srRNA designed to identify Escherichia coli. Four different types of samples we prepared: i) bacterial suspension, ii) bacterial DNA extracted by the phenol – chloroform method, iii) bacterial lysate with lysis buffer and iv) bacterial DNA produced by the boiling method. All four samples were spotted on separate DBC cards and dried at room temperature. After periods of 3, 5 and 7 months, Escherichia coli samples were checked for DNA stability with two molecular techniques, conventional PCR with 1, 2 and 3 disks as a source of bacterial DNA (1 mm diameter) and Real-Time PCR. Results: Data showed that DNA stability was maintained after 7 months on a DBC disk, even using only one disk as a DNA source. The bacterial suspension was the best method for long-term storage of Escherichia coli DNA on the DBC Card. Conclusion: In traditional methods for storing sample, DNA quality reduces after freezing and thawing. However in the DBC method, DNA quality was maintained for a long duration. Advantages of the DBC method are easy sample handing, low cost, faster extraction, and reduced individual and environmental contamination.

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In this study, forced convective heat transfer and pressure drop behavior of multi walled carbon nanotubes (CNT)-water nanofluid were evaluated under constant heat flux in a circular tube. For this purpose, first, homogeneous aqueous suspension of CNT using gum Arabic (GA) surfactant was prepared in concentrations 0.05%, 0.1% and 0.2% wt. Then, the above mentioned nanofluids were evaluated in Reynolds number range of 800-2000 under constant heat flux. The results indicate a significant increase in convective heat transfer coefficient of nanofluids with the addition of small amounts of CNT in deionized water. Also, heat transfer coefficient is enhanced with increasing concentration and Reynolds number. However, the effect of increasing concentrations of CNT is higher than the increase in Reynolds number. In addition, the pressure drop data on the different concentrations and Reynolds numbers are also investigated. At low weight concentrations of CNT, the deal of pressure drop of nanofluids containing CNT and base fluids is approximately similar and the gap between them is negligible. This means that no extra pump power is required for low concentration CNT/water nanofluid. The maximum increase in heat transfer coefficient is 42.8%, which occurred at Re=2027, and a concentration of 0.2% wt.

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There are different methods for the preparation of infusions that can affect the properties of these infusions. The usual method for preparing of infusion is a maceration method that is made using boiling water and a filter. The aim of this study was to prepare marjoram infusion and evaluate the effect of the method of maceration and ultrasonic for different times (5 and 15 min) on phenolic, compounds, free-radical scavenging capacity (DPPH) and heavy metals (arsenic, lead and Cadmium) in the form of a completely randomized design. The results of data analysis obtained from the experiment showed that the preparation method of infusion had a significant effect on all the parameters of the study. The results showed that the amount of phenolic compounds, free-radical scavenging capacity (DPPH) and heavy metals (arsenic, lead and Cadmium)  increased by increasing the time of both processes (ultrasound and maceration). On the other hand, the use of ultrasound waves was found to increase the usefulness of the compounds and also the heavy metals in the marjoram infusion. The total amount of heavy metals measured in this study was less than the standard defined for heavy metals for plant and fruit infusions. Finally, according to the results of this research, it can be stated that using 15 min ultrasound was the best method for making marjoram infusion.
 

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Objective: Breast cancer is considered a heterogeneous disease, characterized by different biological and phenotypic features which make its diagnosis and treatment challenging. We have sought to investigate the expression levels of key components of the Hedgehog signaling pathway, correlation between the signal transducer Smo, and clinicopathologic features (lymph node metastasis and metastasis stage) in invasive breast carcinoma. Also, we examined the inverse correlation between expression levels of Smo and Claudin-1 (an important gene involved in cell tight junctions).
Methods: In this case-control study, we assessed 36 pairs of tumor and adjacent normal tissue specimens obtained from patients with invasive ductal breast carcinoma. The expression levels of key components of Hedgehog signaling (Smo, Gli1 and Ptch), Claudin-1, E-cadherin, and MMP2 were measured by qRT-PCR. The correlations between Smo expression with some clinicopathologic parameters were also analyzed.
Results: We found up-regulation of Hedgehog signaling in invasive breast carcinoma samples compared to normal adjacent tissues.  Upregulation of the signal transducer Smo correlated with tumor stages and lymph node metastasis of the breast tumors. Interestingly, this correlation was affected by the expression of Her2. A significant correlation existed between expression levels of the signal transducer Smo and Claudin-1, E-cadherin as an epithelial cell marker, and MMP2 as a metastasis-related gene in advanced metastatic tumor samples.
Conclusion: Taken together, our study revealed a new layer of molecular complexity which should be considered in the management of patients with invasive breast carcinoma. The results suggested a key role for Hedgehog signaling in invasive breast carcinoma. In terms of the inverse correlation between expression levels of Claudin-1 and Hedgehog signaling, Claudin-1 could serve as a candidate gene in diagnostic studies. Thus, its clinical significance should be further clarified.

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abstract: The independence is relative, in spite of separation of powers, in Islamic Republic of Iran. Importantly, mutual support among government agencies is to allow the service to be more provided to the country and also prevent conflicts or illegal rebellions of the institutions against each other. Lack of proper interaction between governmental institutions led to acting and trading, and this is a disaster. The main objective of this paper is to draw the desired pattern of relationships between sovereign institutions within the framework of the constitution. The main criterion which is essential to be considered in the development of relations between institutions of governance is "check and balance". So, the first step is to try to prove the implications of the relationship between sovereign entities in accordance with the constitution using content analysis, and also provide a consensus by using Delphi technique. After condition stipulating in line with the constitution, issues or problems between the sovereign institutions were reviewed to achieve the desired study pattern using approach of focus group and finally a model with greater transparency in the check and balance mechanism of the sovereign institutions on each other was provided.

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In this study, the effect of enzyme type (alcalase and pepsin) and process time (50-300 minutes) on the degree of hydrolysis and antioxidant indices including free radical scavenging of DPPH, ABTS, hydroxyl, reducing power, and chelating activity of Iron and copper ions were evaluated for navy bean protein (Phaseolus vulgari L.). Also, the composition of amino acids (hydrophobic and antioxidants types) and structural properties (FTIR) of primary protein and hydrolysates were investigated. The results showed that enzymatic hydrolysis improves antioxidant properties. Also, the composition of amino acids has a significant effect on antioxidant activities. On the other hand, the type of enzyme and the time of the hydrolysis process affected the degree of hydrolysis and the antioxidant activity of the hydrolysates. Thus, the highest percentage of free radicals scavenging of DPPH (82.4%), ABTS (58.3%), reducing power (0.97), hydroxyl radical scavenging (57.5%), and chelation of Fe (53.7%) and Cu ions (12.1%) were affected by the type of enzyme and process time. Among different treatments, the highest value of these indices (except copper ion chelating) was related to hydrolysates with alcalase. Structural properties of white bean protein were evaluated and enzymatic hydrolysis caused changes in the amide regions (I and II) as well as exposure to some hydrophobic-buried groups. The results of this study indicated the positive effect of enzymatic hydrolysis on the production of antioxidant hydrolysates that can be used in the food industry.

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The main goal of the current study was to isolate and characterize endospore-forming soil bacteria that produce parasporal crystalline proteins against larvae of Lepidoptera insects during stationary phase of growth. Two different methods were applied for the isolation of spore-forming strains. Polymerase Chain Reaction (PCR) was used for the characterization of cry gene content of the isolated strains and 16S rRNA sequencing was carried out to identify bacterial strains. Efficiency of the isolates as insecticide was evaluated in a bioassay experiment using insect's larvae. Regarding presence of parasporal inclusion crystals and SDS-PAGE patterns, 10 bacterial strains were isolated from about 200 soil samples. Analysis of crystal-spore mixtures with SDS-PAGE showed a broad range of proteins with molecular weight between 11-230 kDa. Data from PCR analysis indicated that only two isolates (RIPI6 and RIPI18) may contain cry3 gene. Isolate RIPI21 and reference strain (Bacillus thuringiensis subsp. kurstaki) were positive for cry1 gene. 16S rRNA gene sequences of all isolates showed at least 96% sequence match with B. thuringiensis strains deposited in the GenBank. The results of bioassay experiments showed the efficacy of strains RIPI7, 10 and 22 on killing of larvae of both Anagasta kuehniella Zeller and Plutella xylostella Curt. It is concluded that there are some bacterial candidates for biological control of major agricultural pests in north of Iran.