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In this paper, the effect of gas and liquid inlet superficial velocities and distance from upstream on slug frequency is studied experimentally. Empirical correlations are also presented based on the obtained results. The tests are conducted for liquid holdup αl= 0.75 and three distances from inlet in a long horizontal channel made of Plexiglas with dimensions of 510 cm2 and 36m length in Multiphase Flow Lab. of Tarbiat Modares University. The superficial liquid and air velocities rated as to 0.11-0.56 m/s and 1.88-13 m/s, respectively. The obtained results show that slug frequency is dependent to superficial liquid velocity directly. Slug frequency decreases with slip ratio increase. Slug frequency has strong dependency on superficial liquid velocity and increases monotonically with it. However, superficial gas velocity has damping effect on slug frequency. As slug moves towards downstream, slug frequency will be decreased but slug velocity will be increased.