In this study, we tried to have investigation of elastic properties of Prefoldin nano-actuator on the microscopic scale. Prefoldin is a molecular chaperone that prevented the aggregation of misfolded proteins and it has been shown that it can also serve as a Nano-actuator (drug delivery). To this end, steered molecular dynamics simulations have been used, which investigate the theory of spring constant in the molecular test based on the theory of two springs in series. The results expressed in form of young’s modulus. The results show that Prefoldin nano actuator exhibit different behaviors at different pulling rates and to what extent of tension, each tentacle of this nano actuator remains stable. The resulting Young's modulus for the Prefoldin chains was obtained at a rate of (3-3.3 ± 0.01 Gpa). By providing the complete understanding of mechanical properties of Prefoldin nano actuator, it is possible to exact investigating of Prefoldin nano actuator applications in intelligent drug delivery and capture the pathogenic cargos.