High temperatures and different properties of entering gas into the turbine of a gas turbine cycle can decrease its performance. Considering the complexity of the flow distribution inside the turbine, three-dimensional analysis to find out the flow and temperature field in the turbine stages is very important. As time passing the increasing of the roughness of blades is unavoidable. The aim of this paper is investigation of the blades roughness effects on flow field and efficiency of gas turbine with numerical calculations. In this research, a two-stage turbine is modeled in the form of three-dimensional and the results are validated with experimental data. Then the effects of blades roughness on flow field and performance of turbine in five pressure ratios is investigated. Also, in order to determine the role of stators and rotors in decreasing the turbine efficiency, in a special roughness, the first and second stators and then corresponding rotors have separately been examined and then this phenomenon affected on blades simultaneously. Results showed that the efficiency drop by applying all together on the turbine stage is approximately equal to summation of efficiency drops by applying separately.