Nowadays, the building ventilation is an essential process, due to need of improving the air quality and thermal comfort conditions for occupants. Providing the mentioned conditions is more complex for crowded and larger spaces. In this study, the effects of air change rate per hour (ACH) on thermal comfort, indoor air quality and energy consumption in an amphitheater with under floor air distribution system have been investigated by using the computational fluid dynamics and Open Foam numerical solver. For this issue, an amphitheater with 50 occupants has been modeled under the conditions that the air inlet diffusers located in front of seats. Also, the air change rate per hour is assumed to be 5, 10 and 15. For better comparison between the results, inlet air temperature is controlled until the mean of thermal comfort index (TSENS) in the occupied zone equals to zero. The results indicate that for air change rates of 15, 10 in comparison with ACH of 5, the CO2 concentrations in the occupied zone are respectively reduced about 36 and 46 percent and so the indoor air quality is improved. On the other hand, the energy consumption is increased about 28 and 69 percent, respectively. Also, based on the results, by increasing the ACH rate, the draft local discomfort is significantly increased and can be reached at the amount of 15%.