Modares Mechanical Engineering

Modares Mechanical Engineering

Two-dimensional modeling of bio-particles separation by Inertia in micro-channel

Authors
Sohil Arbabi 1
mostafa mafi 1
Madjid Soltani 2
1 Department of Mechanical Engineering, Imam Khomeini International University, Qazvin, Iran
2 Faculty of Mechanical Engineersing, K. N. Toosi University of Technology, Tehran, Iran
Abstract
Early diagnosis of hypertensive diseases such as cancer plays an essential role in preventing disease progression. The main cause of death from cancer is the reappearance of the disease due to the release of tumor cells in the blood of the patient. Among the various methods that have been devised for monitoring blood in recent years, the techniques based on micro-scale flow have specially been considered. The development of these methods has led to the emergence of microfluidics laboratories on the chips, which their main advantages are low prices and simplicity. Since the particles’ sizes are different in the flow of blood, the direction of these particles in the micro-channels will vary due to the different forces, and therefore they can be analyzed to the design of bio-microchips. In the present study, a two-phase flow containing spherical particles with the dimensions of blood cells was considered, and the forces affecting the particles of this current, including the lift forces and drag forces, were studied using COMSOL software. For this purpose, a micro divergent channel was designed and the effect of ratio of the outlet width to the inlet width (Aspect Ratio) as an effective geometric parameter in the biological particle separation was analyzed. The study of the effect of particle dimensions and various geometric parameters of the channel on bio-particles separation are the main goals of this research. The results show that by increasing the Aspect ratio, focusing of the larger particles would increase at the outlet of micro-channel.
Keywords
Micro fluidics
Bio particles Separation
Lab on a chip
Inertia
[1] M. Cristofanilli, Circulating tumor cells, disease progression, and survival in metastatic breast cancer, Seminars in Oncology, Vol. 33, No. 9, pp. 9–14, 2006.
[2] S. Paget, The distribution of secondary growths in cancer of the breast, The Lancet, Vol. 133, No. 3421, pp. 571–573, 1889.
[3] C. Alix-Panabières, K. Pantel, Circulating tumor cells: Liquid biopsy of cancer, Clinical Chemistry, Vol. 59, No. 1, pp. 110–118, 2013.
[4] K. Pantel, C. Alix-Panabières, Circulating tumour cells in cancer patients: Challenges and perspectives, Trends in Molecular Medicine, Vol. 16, No. 9, pp. 398–406, 2010.
[5] M. P. Lee, M. J. Padgett, Optical tweezers: A light touch, Journal of Microscopy., Vol. 248, No. 3, pp. 219–222, 2012.
[6] K. Dholakia, T. Čižmár, Shaping the future of manipulation, Nature Photonics, Vol. 5, No. 6, pp. 335–342, 2011.
[7] M. Hejazian, W. Li, N. T. Nguyen, Lab on a chip for continuous-flow magnetic cell separation, Lab on a Chip, Vol. 15, No. 4, pp. 959–970, 2015.
[8] J. S. Jeong, J. W. Lee, C. Y. Lee, S. Y. Teh, A. Lee, K. K. Shung, Particle manipulation in a microfluidic channel using acoustic trap, Biomedical Microdevices, Vol. 13, No. 4, pp. 779–788, 2011.
[9] P. K. Wong, C. Y. Chen, T. H. Wang, C. M. Ho, Electrokinetic bioprocessor for concentrating cells and molecules, Analytical Chemistry, Vol. 76, No. 23, pp. 6908–6914, 2005.
[10] F. Gielen, A. J. deMello, J. B. Edel, Dielectric cell response in highly conductive buffers, Analytical Chemistry, Vol. 84, No. 4, pp. 1849–53, 2012.
[11] N. Pamme, Continuous flow separations in microfluidic devices, Lab on a Chip, Vol. 7, No. 12, pp. 1644, 2007.
[12] T. E. Kagalwala, J. Zhou, I. Papautsky, Continuous size-based separation of Microparticles in straight channels, Proceedings of 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Groningen, Netherlands, 3-7 October, 2010.
[13] J. Zhou, P. V. Giridhar, S. Kasper, I. Papautsky, Modulation of aspect ratio for complete separation in an inertial microfluidic channel, Lab on a Chip, Vol. 13, No. 10, pp. 1919-1929, 2013.
[14] M. Yamada, M. Nakashima, M. Seki, Pinched flow fractionation: Continuous size separation of particles utilizing a laminar flow profile in a pinched microchannel, Analytical Chemistry, Vol. 76, No. 18, pp. 5465–5471, 2004.
[15] J. M. Martel, M. Toner, Inertial focusing in microfluidics, Annual Review of Biomedical Engineering, Vol. 16, No. 1, pp. 371–396,. 2014.
[16] H. Amini, W. Lee, D. Di Carlo, Inertial microfluidic physics, Lab on a Chip, Vol. 14, No. 15, pp. 2739-2761, 2014.
[17] G. Segré, A. Silberberg, Behaviour of macroscopic rigid spheres in Poiseuille flow Part 1. Determination of local concentration by statistical analysis of particle passages through crossed light beams, Journal of Fluid Mechanics, Vol. 14, No. 1, pp. 115-135, 1962.
[18] J. Zhou, I. Papautsky, Fundamentals of inertial focusing in microchannels, Lab on a Chip, Vol. 13, No. 6, pp. 1121-1132, 2013.
[19] J. Zhang, S. Yan, D. Yuan, G. Alici, N. T. Nguyen, M. Ebrahimi Warkiani, W. Li, Fundamentals and applications of inertial microfluidics: A review, Lab on a Chip, Vol. 16, No. 1, pp. 10–34, 2016.
[20] B. P. Ho, L. G. Leal, Inertial migration of rigid spheres in two-dimensional unidirectional flows, Journal of Fluid Mechanics, Vol. 65, No. 2, pp. 365- 400, 1974.
[21] E. S. Asmolov, The inertial lift on a spherical particle in a plane Poiseuille flow at large channel Reynolds number, Journal of Fluid Mechanics, Vol. 381, No. 4, pp. 63–87, 1999.
[22] J. A. Schonberg, E. J. Hinch, Inertial migration of a sphere in Poiseuille flow, Journal of Fluid Mechanics, Vol. 203, No. 6, pp. 517–524, 1989.
[23] C. Liu, C. Xue, J. Sun, G. Hu, A generalized formula for inertial lift on a sphere in microchannels, Lab on a Chip, Vol. 16, No. 5, pp. 884–892, 2016.
Modares Mechanical Engineering
Volume 18, Issue 1
March 2018
Pages 239-246