Modares Mechanical Engineering

Modares Mechanical Engineering

Theoretical and Experimental Simulation of Young Modulus Extraction of Breast MCF-10 Cells Using Atomic Force Microscope

Document Type : Original Research

Authors
Moein Taheri
mahdi mirzaluo
Department of Mechanical Engineering, Arak University, Arak, Iran
Abstract
Mutations in DNA and the development of mutated genes that are inherited or acquired during a personchr('39')s lifetime can cause cancer. This type of disease causes the loss of normal control of cell growth and proliferation. Breast cancer, with its prevalence in both men and women and the higher incidence of women in the female population, is one of the most important cancers in the medical community. Appearance changes in the breast, the presence of a lump, and discharge and bleeding from the nipple are signs of breast cancer. Targeted treatment of this disease reduces the complications of treatment methods. Also, recognizing the mechanical properties of the cell, such as the Youngchr('39')s modulus, and examining the changes caused by cancer in these properties will make treatment more efficient and help the pharmaceutical sciences. For this purpose, in this paper, the MCF-10 breast cell has been studied using atomic force microscopy and nanomanipulation method. Atomic force microscope is one of the efficient tools in the structural studies of biological particles with the possibility of producing images of soft tissues under different environmental conditions and in a non-destructive manner. Chung, chen and brake contact are the models used in the simulation. Finally, with the simulations performed, the Young modulus of 1200 Pa is considered for this cell. Also, considering the comparisons made with experimental work, the chen contact model has been introduced as the optimal model for extracting cellular properties.
Keywords
Young modulus
MCF-10 cell
Atomic Force Microscopy
contact models
Breast Cancer

Subjects

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Modares Mechanical Engineering
Volume 22, Issue 1
December 2021
Pages 37-45